Monitoring of Metal Elements in CHO Cell Culture Supernatant Using ICPMS-2030

User Benefits

- Simultaneous analysis is possible for metal elements in CHO cell culture supernatant. - The cell culture supernatant can be analyzed by using the ICPMS-2030 with only dilution.

Introduction

The active pharmaceutical ingredients (API) of antibody drugs are mainly produced by culturing CHO (Chinese hamster ovary) cells. In recent years, it has been reported that the metabolism of cultured cells and the quality of antibodies produced are affected by changes in the concentration of metal elements in the culture medium. For example, it is known that the lactate metabolism of CHO cells changes according to the concentration of Cu in the culture medium, and the glycosylation of antibodies changes according to the Mn/Zn ratio in the culture medium. Therefore, in order to maintain the quality of antibody drugs, it is important to simultaneously monitor changes over time in the concentrations of multiple metal elements in the medium and culture supernatant. The measurement examples of the medium and culture supernatant by AAS were shown in the Application News A634 and A651. On the other hand, ICP-MS is considered suitable for trace elements analysis and simultaneous monitoring of multiple elements . The Application News 01-00372 showed that the spike recovery rate and the long-term stability of culture medium measurements confirmed that the ICPMS-2030 is useful for medium measurements. In this report, we introduce an example of monitoring metal elements with the ICPMS-2030 by diluting the culture medium/culture supernatant of CHO cells.

August 31, 2022 GMT