Measuring Bile Acid Levels in Human Plasma Using a Triple Quadrupole Mass Spectrometer

User Benefits

- Comprehensive measurement of bile acids in plasma - Stable analysis in the long term without system tuning

Introduction

Bile acids play an important role in fat absorption in the small intestine and are also involved in regulating cholesterol metabolism through the conversion of cholesterol into bile acids. Primary bile acids are produced by cholesterol catabolism in the liver, most of which conjugate with taurine or glycine to produce conjugated bile acids. Some primary bile acids are also modified later by intestinal bacteria to produce secondary bike acids. Total bile acid concentration in peripheral blood is a marker for liver dysfunction in humans and blood enzyme activity (ALT, AST, etc.) and total bile acid (TBA) tests are routine. Monitoring levels of multiple bile acids individually may also allow the identification of various liver disorders, and based on this the simultaneous analysis of bile acids has been attracting interest. This article describes a quantitative analysis of 22 bile acids in human plasma performed using 9 internal standards. Analysis was performed using the LC/MS/MS Method Package for Bile Acids that contains various LC and MS setup conditions optimized for LC/MS/MS. The analytical system used was the LCMS-8060 high- performance liquid chromatograph-mass spectrometer.

December 25, 2022 GMT