MALDI imaging of animal tissues and fingermarks using the MALDI-8020 benchtop linear MALDI-TOF mass spectrometer

User Benefits

- A benchtop linear MALDI-TOF MS is applied to various MS imaging applications showing speed and ease of operation. - MS imaging at 30 μm spatial resolution was demonstrated in the rat brain and the fingermark data. - Highly reproducible MS imaging provided by iMLayerTM and MALDI-8020.

Introduction

MS imaging is one of the growing applications in mass spectrometry. While the state-of-art instruments, which have high spatial resolution, high MS resolving power capabilities, have been applied to imaging applications, some demands for miniaturization of instrument and high accessibility are increasing in various scientific/industrial fields, in which a high- performance instrument is not necessary. In this article, we report the use of a benchtop MALDI-TOF MS for the MS imaging application. The MALDI-8020 and iMLayer, our commercial imaging sample preparation device were able to generate MS images of rat brain and fingermarks at 30 μm spatial resolution with quick and easy operation. The accessible MS imaging workflow presented in this article, consisting of a benchtop MALDI-TOF MS, matrix deposition methods, and software, could be applicable to various applications.

January 26, 2023 GMT