Cell Culture Profiling Analysis with LC-MS/MS and Bioanalyzer for Antibody Production in Chinese Hamster Ovary (CHO) Cell System

User Benefits

- LC-MS/MS cell culture profiling method package allows simultaneously analysis of up to 125 targeted nutrients and metabolites in cell culture medium. - Graphical trend plots of detected targets enable to view the changes of metabolite concentrations with cell culture time directly. - Trends of metabolite changes from LC-MS/MS are highly correlated with the trends observed from CEDEX Bio Analyzer.

Introduction

Chinese hamster ovary (CHO) cells are widely used in the biopharmaceutical industry as the workhorse to produce therapeutical recombinant protein including monoclonal antibody (mAb). Monitoring the abundance of nutrients and metabolites during CHO cell culture provides important insights into the process performance since cell growth and protein product quality can be impacted by the depletion of key nutrients or accumulation of cellular metabolism by-products. Thus, it is worthwhile to perform a comprehensive analysis on cell culture medium as such knowledge can aid in developing a well- balanced media formulation and an optimized feeding strategy for biopharmaceutical production. With conventional sensors on bioreactor or standalone bioanalyzers, no more than 10 metabolites can be monitored in a single run. Furthermore, expensive reagent kits may be needed and some of these kits have short shelf life once being opened. This puts additional stress in experiment planning and resource allocation. Herein, we monitored the cell culture process in mAb production by an LC-MS/MS approach using Shimadzu cell culture profiling method package which enables analysis of 125 media components in a single run of LC- MS/MS analysis.

February 9, 2023 GMT