Quick Profiling and Identification of Natural Product Components in Honeysuckle Flower by DDA Method on LCMS-9030 with LabSolutions Insight Explore

User Benefits

- Effective data-dependent acquisition (DDA) with preferred ions on LCMS-9030 Q-TOF was used for profiling and identification of main components in Honeysuckle Flower extract. - LabSolutions Insight Explore s/w provides highly-flexible and in-depth data analysis tools for targeted and un-targeted screening, identification and structure elucidation using Analyze, Screen, Precursor, Library Search, Formula and Assign.

Introduction

The use of LC-HRMS such as LC-Q-TOF for profiling and identification of natural products and herbal medicines has become an important and effective approach in quality control, research and discovery of new active compounds. The accurate mass (1 ppm accuracy) and rich MS/MS data acquired by data-dependent acquisition (DDA) or data-independent acquisition (DIA) allow researcher to perform targeted and/or untargeted screening as well as identification of unknown components in an extract sample. In this article, we describe DDA analysis approach applying to an extract sample of Honeysuckle flower, Lonicera japonicae, a traditional Chinese medicine (TCM). We demonstrate the in-depth data analysis using a comprehensive software, LabSolutions Insight Explore, for screening and identification using various tools including Analyze, Screen, Precursor, Formula Predictor, Library Search and Assign. Lonicera has been used as herbal remedies to treat illness since ancient time. It has been documented in China Pharmacopoeia (2005 Edition). It is of great interest to profile and identify the key components present in Honeysuckle flower extracts for quality control and research purpose.

July 6, 2023 GMT