Determination of Counter Ions of Synthetic Peptides Using Ion Chromatograph

User Benefits

- The purity of synthetic peptides can be confirmed using ion chromatograph. - Trifluoroacetic acidand chloride ions can be determined simultaneously. - The system can be selected according to the purpose, such as a suppressor type when used in conjunction with high-sensitivity analysis such as the impurity measurement of inorganic anions in pharmaceuticals, or a non-suppressor type with a simple equipment configuration when focusing on cost performance.

Introduction

The middle molecule drug is attracting attention as a next generation drug discovery modality. The middle molecule drugs have a smaller molecular weight than biopharmaceuticals, and there are oligonucleotide therapeutics using oligonucleotides and peptide therapeutics with peptide skeletons. Among them, peptide therapeutics have the advantage that they can be produced at low cost, easily taken into cells due to their small molecular weight, and can suppress degradation when taken into the body by adopting a specific conformation. Since trifluoroacetic acid (TFA) is used to extract the synthesized peptides from the stationary phase, the recovered peptides are converted into peptide TFA salts in which TFA is ionically bonded. The weight of the lyophilized peptide includes the weight of this TFA, which greatly affects the actual peptide content. In addition, TFA may affect bioavailability, and it is necessary to replace it with a salt such as hydrochloride. Therefore, it is essential to quantify the counter ion to confirm the purity of synthetic peptides. In this paper, we report an example of counter ion analysis using an ion chromatograph (IC) using crude purified linear and cyclic synthetic peptides.

February 13, 2025 GMT