Analysis of Methionine Sulfone and Cysteic Acid Using Automated Pretreatment Functions

User Benefits

- This method can provide significantly short analysis time compared to the post-column derivatization method generally used for amino acid analysis. - This method without manual pretreatment is easy to use because complicated derivatization process is well automated. - Twenty proteinogenic amino acids can be analyzed as well using the same column and the derivatization reagents as this method.

Introduction

Hydrochloric acid hydrolysis is commonly used as a pretreatment when analyzing amino acids. However, sulfur-containing amino acids such as methionine, cysteine, and cystine are oxidized in the process. For accurate determination of these amino acids, methionine and cysteine/cysteine should be oxidized preliminarily to methionine sulfone and cysteic acid respectively by performic acid treatment, followed by hydrolysis procedures to determine methionine sulfone and cysteic acid. The post-column derivatization method is often used for the analysis of methionine sulfone and cysteic acid. However, this method is not suitable for high-speed analysis due to cation exchange column. On the other hand, the pre-column derivatization method for amino acids provides very easy analytical operation because of automatic derivatization of the complicated process. This article introduces the analysis of methionine sulfone and cysteic acid using the automatic pretreatment function of LC-2050C integrated high-performance liquid chromatograph.

May 20, 2025 GMT