Simultaneous Analysis of Synthetic Colorants, Including Red No. 3, Using Integrated LC System

User Benefits

- The integrated LC system i-Series enables simultaneous analysis of 12 synthetic colorants, including Red No. 3. - The organic silica hybrid-based Shim-pack, NovaCore C18-HB provides a wide pH tolerance range from 1 to 12 for both the mobile phase and sample solvent. - LabSolutions MD,dedicated software for supporting method development, enables efficient exploration of separation conditions.

Introduction

Food colorants can be categorized into natural and synthetic types. The availability of synthetic colorants varies across countries, depending on regulatory frameworks. In Japan, twelve synthetic colorants are currently approved as food additives, and their safety can be assessed through qualitative and quantitative analysis using liquid chromatography. Recently, the U.S. Food and Drug Administration announced a prohibition on the use of Red No. 3, one of these twelve colorants, in food products, further increasing interests regarding the safety of synthetic colorants. This study presents an example of simultaneous analysis of twelve synthetic colorants using the integrated LC system i-Series in combination with the organic silica hybrid core-shell column, Shim-pack NovaCore C18-HB. The optimization of separation conditions was performed efficiently using LabSolutions MD, a dedicated software for supporting method development. Furthermore, the applicability of the optimized method is demonstrated through the analysis of real samples.

August 31, 2025 GMT