Rapid Structural Elucidation of Lipids Including C=C Positions Using Dual-Polarity CID/OAD in a Single LC-MS Run

User Benefits

- Combining positive/negative polarity switching and simultaneous CID/OAD analysis acquires all the information required for detailed structural analysis of lipids in a single LC-MS analysis run. - Carbon-carbon double bond positions in co-eluted lipid structural isomers are also determined.

Introduction

Lipids are essential for life and serve a variety of physiological functions, such as cell wall components, signal transmitters, energy storage, and epithelial barrier formation. Disruption of lipid homeostasis is implicated in many diseases, and non-targeted lipidomics is a powerful multi-omics research technique for elucidating disease pathologies and identifying specific biomarkers that show lipid changes in the body. Carbon-carbon double bond (C=C) position lipid isomers have a significant impact on enzyme selectivity, the physical properties of biomembranes, and other biological functions. However, these isomers are not easily distinguishable by collision-induced dissociation (CID) analysis. Analytical techniques capable of distinguishing double bond position isomers are extremely important in understanding the mechanisms of lipid homeostasis in different tissues and how these mechanisms change with various diseases.

November 11, 2025 GMT