Multifaceted Spectrophotometric Analysis of Vitamins and Vitamin-like Substances

User Benefits

- Ultraviolet-visible spectrophotometers can measure the light absorption properties, ranging from ultraviolet to visible light, of liquids, and spectrofluorophotometers can measure the fluorescent light properties of liquids and powders. - Fourier transform infrared spectrophotometers and Raman spectrophotometers enable easy qualitative analysis of substances that are in liquid or powdered states. - Spectrophotometric properties can be investigated easily by using a variety of spectrophotometric methods, depending on the sample.

Introduction

Vitamins help to maintain proper bodily functions, but almost none of them can be synthesized within the body, so they must be obtained from foods. Generally, vitamins can be classified as either water-soluble vitamins, which dissolve easily in water, or fat-soluble vitamins, which do not. Vitamin-like substances are nutrients that serve a similar function to vitamins and can be synthesized within the body. Both vitamins and vitamin-like substances, which are essential to humans, require analytical instruments to determine which substances contain them and to what degree. Information about simultaneous analysis of water-soluble and fat-soluble vitamins is included in Analytical Solutions for Food Development. This article provides an overview of measuring vitamins and vitamin-like substances using the spectrophotometric instruments shown (a UV-VIS spectrophotometer, a spectrofluorophotometer, a Fourier transform infrared spectrophotometer, and a Raman spectrophotometer), and it describes some examples.

December 2, 2025 GMT