Total RNA Quality Evaluation by RNA Integrity Index (RII) Using MultiNA II

User Benefits

- Possible to automate the electrophoresis operation - Automatically calculates RNA Integrity Index (RII) needed to assess the quality of Total RNA - Quick and easy measurement of multiple samples

Introduction

Total RNA extracted from biological samples is the basic material for various experiments including sequencing analysis (next generation, Sanger), and the yield and quality of total RNA are affected by the original sample collection and pretreatment conditions. The quality of the total RNA obtained by extraction will greatly determine the success or failure of subsequent experiments. Traditionally, total RNA quality has been evaluated by ultraviolet (UV) spectrophotometry and electrophoresis (denaturing agarose gel electrophoresis). With the advent of electrophoresis instruments, the RIN (RNA Integrity Number) obtained by Agilent Technologies' Bioanalyzer in particular has long been used as the de facto standard for total RNA quality assessment. Based on this result, a decision is made whether to use the obtained total RNA for subsequent experiments or to reprepare it. Shimadzu's microchip electrophoresis system MultiNA II MCE-301 can calculate RII (RNA Integrity Index), an index for evaluating the quality of total RNA from electrophoresis results. Here we introduce the correlation between RII, a total RNA quality index calculated from the results of electrophoresis of total RNA from various tissues by MultiNA II, and RINe (RNA Integrity Number equivalent) obtained by 4150 TapeStation System (Agilent Technologies) will be introduced.

January 27, 2026 GMT