Quality Evaluation of mRNA by Electrophoresis

User Benefits

- Electrophoresis operations can be performed almost entirely automatically. - Electrophoresis completes in approximately 100 seconds per analysis. - It is possible to detect impurities such as mRNA degradation products and off-target RNA.

Introduction

mRNA vaccines and pharmaceuticals are prepared through processes including synthesis by transcription, addition of the Cap structure, and purification. Methods for conveniently confirming the purity and yield of mRNA during the purification process are sought, and high-performance liquid chromatography (HPLC) and electrophoresis are commonly used. Microchip electrophoresis offers a simpler and faster method for obtaining electrophoresis results compared to capillary electrophoresis, despite having a shorter separation length and thus lower resolution. We present results verifying the applicability of microchip electrophoresis analysis for confirming the purity of target mRNA and detecting impurities.

February 11, 2026 GMT