Rapid and Simple Analysis of LNP-Encapsulated mRNA Using a Microchip Electrophoresis System

User Benefits

- Automates gel preparation, sample application, and data acquisition for nucleic acid electrophoresis. - Enables rapid and simple measurement of LNP-encapsulated mRNA samples without purification. - Provides highly sensitive mRNA analysis.

Introduction

Recently, medical technology utilizing mRNA has advanced rapidly. In addition to infectious disease vaccines, mRNA is gaining attention in fields such as cancer vaccines and gene therapy. Since mRNA is a large, negatively charged molecule with poor cell membrane permeability, delivery technology is essential for efficient transport into cells. Currently, Lipid Nanoparticles (LNPs) are primarily used as Drug Delivery Systems (DDS). This technology protects mRNA from extracellular degradation and enables efficient intracellular transport. LNP-encapsulated mRNA (mRNA-LNP) formulations are expected to serve as treatments for various diseases; however, quality control in their manufacturing and storage has become a critical challenge. The quality of mRNA-LNP formulations directly impacts their safety, efficacy, and stability. Therefore, establishing appropriate analytical methods for quality evaluation is essential for improving product reliability and ensuring compliance with regulatory standards. Methods for confirming mRNA purity include agarose gel electrophoresis and capillary electrophoresis; however, these can be disadvantageous due to the time required for preparation and analysis, or insufficient resolution. In contrast, the Microchip Electrophoresis System MultiNA II automates the entire process from gel preparation to sample loading and data analysis. It enables rapid analysis with a migration time of approximately 100 seconds per sample. This application presents an example of simple and rapid analysis of mRNA-LNP using the MultiNA II without the need for pretreatment.

March 17, 2026 GMT