Multiresidue Pesticides Analysis in Solvent Extract of Annatto (Natural Color Additive) Using GCMS-TQ8050 NX

User Benefits

- Accurate and reproducible trace level quantitation of pesticides in highly pigmented complex matrix using Ultra-fast GCMS-TQ8050 NX and QuEChERS extraction - Single-laboratory partial validated method for LOQ determination meeting regulatory guidelines - Ease of instrumental method optimization using GCMS Smart Pesticides Database Ver.2 from Shimadzu Corporation

Introduction

Annatto is an orange-red condiment and food colorant derived from the seeds of the achiote tree (Bixa Orellana) . It is often used to impart a yellow to red-orange color to foods, but sometimes also for its flavor and aroma. Similar effects can be obtained by extracting some of the color and flavor principles from the seeds, which are then added to the food. Annatto and its extracts are now widely used in an artisanal or industrial scale as a coloring agent in many processed food products, such as cheeses, butter, baked goods, potatoes, snack foods, breakfast cereals, smoked fish etc. In these uses, Annatto is a natural alternative to synthetic food coloring compounds. Owing to its large culinary uses and other diverse applications, use of chemical pesticides for its production in large quantities is imperative. Dye extraction process may result in concentration of pesticides and in turn contribute to adverse impact on human health when incorporated in various preparations. Hence quantitation of residual pesticides in Annatto extract becomes very important. As the oleoresin is a complex matrix, it is required to develop a rugged, sensitive and efficient method for extraction of residual pesticide and its analysis. This study reports a highly sensitive method for simultaneous quantification of multiple pesticides in highly pigmented complex matrix of Annatto using modified QuEChERS with triple quadrupole gas chromatography (GC-MS/MS) system.

March 29, 2026 GMT