Characterization of a GalNAc-siRNA Conjugate Using a Quadrupole Time-of-Flight Mass Spectrometer

User Benefits

- The LCMS-9050 quadrupole time-of-flight mass spectrometer and the LabSolutions Insight Biologics analysis software can be used to characterize GalNAc-siRNA conjugates, such as with molecular weight confirmation and sequence analysis. - LabSolutions Insight Biologics enables analysis of multiple oligonucleotide sequences at the same time. Nucleobases, linkers, riboses, and base modifications can be added and removed as required.

Introduction

Among oligonucleotide therapeutics, antisense and siRNA therapeutics are actively studied as new modalities for treating genetic and intractable diseases. Oligonucleotide therapeutics modified with N-acetylgalactosamine (GalNAc) have attracted attention as drug delivery systems (DDS) designed to enhance the uptake of these therapeutics into the liver. GalNAc binds to the asialoglycoprotein receptor (ASGPR), which is highly expressed in the liver, and is subsequently internalized into hepatocytes. By exploiting this mechanism, GalNAc-modified oligonucleotide therapeutics can be efficiently delivered into hepatocytes. In many cases, a tri-antennary GalNAc (Tri-GalNAc) structure containing three GalNAc residues is used as a highly efficient ligand for ASGPR. This article describes an example of ion-pair reversed-phase LC/MS analysis for characterization of GalNAc-siRNA conjugates using a quadrupole time-of-flight mass spectrometer.

April 7, 2026 GMT