Ion-Pair-Free Reversed-Phase LC/MS Analysis of siRNA Using a Single Quadrupole Mass Spectrometer

User Benefits

- The LCMS-2050 single quadrupole mass spectrometer and the LabSolutions Insight Biologics analysis software can be used to confirm the molecular weight of siRNA. - The ion-pair-free method does not use ion-pair reagents, so it decreases the risk of instrument contamination and does not need an instrument dedicated to ion-pair regents. - LabSolutions Insight Biologics enables analysis of multiple oligonucleotide sequences at the same time.

Introduction

Among oligonucleotide therapeutics, antisense and siRNA therapeutics are actively studied as new modalities for treating genetic and intractable diseases. Unlike single-stranded antisense, siRNA is double-stranded RNA known to separate into single-stranded RNAs under heated or alkaline conditions. LC/MS analysis of oligonucleotides commonly involves using reversed-phase chromatography with mobile phases containing an alkylamine ion-pair reagent, such as hexylamine or triethylamine. However, the use of ion-pair reagents raises concerns that instrument contamination may affect other analyses. This Application News article describes an example of ion-pair-free reversed-phase LC/MS analysis of siRNA using a single quadrupole mass spectrometer.

April 22, 2026 GMT