Analysis of Duplex siRNA Impurities under Non-Denaturing Conditions Using a Single Quadrupole Mass Spectrometer

User Benefits

- The LCMS-2050 single quadrupole mass spectrometer with LabSolutions Insight Biologics analysis software can be used to confirm the molecular weight of siRNA. - The LCMS-2050 can identify impurities that cannot be separated from target components by LC.

Introduction

Antisense and siRNA therapeutics are oligonucleotide therapeutics actively studied as new modalities for treating genetic and intractable diseases. Unlike single-stranded antisense, siRNAs are duplex RNA molecules known to dissociate into single-stranded RNAs under heated or alkaline conditions. This Application News article describes an example of duplex siRNA impurity analysis by ion-pair reversed-phase chromatography using an LCMS-2050 single quadrupole mass spectrometer. Using a mass spectrometer enables detection of impurities that cannot be separated from FLP by LC.

November 10, 2026 GMT