LC/MS/MS Analysis of Vitamin D3 by the Co-Sense for Impurities System

Introduction

The Co-Sense for Impurities system, as previously described in Application News No. L424, is a complete HPLC configuration that automates the sample pretreatment process in trace level analysis. The earlier article presented an example of high sensitivity detection using analyte extraction and concentration. This system offers analyte concentration and removal of impurities, and therefore comprises 3 flow lines: one for primary separation, another for concentration, and the third for secondary separation. Since a different mobile phase and column can be selected for each flow line, a combination of conditions can be used, including those that are conducive for separation at the primary separation stage and that are appropriate for detection for the secondary separation stage. Here we introduce an example in which Vitamin D₃ capsules were analyzed by LC/MS/MS using such a method.

September 18, 2012 GMT