Shim-pack Velox C18 is a traditional end-capped C18-bonded phase which offers the highest hydrophobic retention of any Shim-pack Velox phases and is applicable to a wide range of applications such as pharmaceutical, food, environmental and clinical and neutrals at moderately low and mid-range pH.
- General purpose column for reversed-phase chromatography
- Highest hydrophobic retention among Shim-pack Velox series
- Compatible with moderately acidic to neutral mobile phases (pH 2-8)
Method Transfer for Cyanocobalamin Analysis within the USP Allowable Adjustment
The assay of cyanocobalamin (a synthetic form of vitamin B12) with 5 μm fully-porous ODS column described in the USP monograph is transferred to a new method with Shim-pack Velox C18 2.7 μm column, within USP allowable adjustments. Analytical time and solvent consumption can be saved with transferred methods while meeting the requirements of system suitability.
|N||System suitability test result
(Requirement: %RSD < 2.0 %)
(5 μm, 4.6 x 150 mm)
|30,000||0.50||5,244||tR: 0.025 %
Area: 0.175 % (n=6)
(2.7 μm, 4.6 x 100 mm)
|tR: 0.035 %
Area: 0.103 % (n=6)
|tR: 0.084 %
Area: 0.220 % (n=6)