Shim-pack Velox C18
HPLC Column

C18
Shim-pack Velox C18 is a traditional end-capped C18-bonded phase which offers the highest hydrophobic retention of any Shim-pack Velox phases and is applicable to a wide range of applications such as pharmaceutical, food, environmental and clinical and neutrals at moderately low and mid-range pH.
- General purpose column for reversed-phase chromatography
- Highest hydrophobic retention among Shim-pack Velox series
- Compatible with moderately acidic to neutral mobile phases (pH 2-8)
Method Transfer for Cyanocobalamin Analysis within the USP Allowable Adjustment
The assay of cyanocobalamin (a synthetic form of vitamin B12) with 5 μm fully-porous ODS column described in the USP monograph is transferred to a new method with Shim-pack Velox C18 2.7 μm column, within USP allowable adjustments. Analytical time and solvent consumption can be saved with transferred methods while meeting the requirements of system suitability.
Column | L/dp | Flow rate (mL/min) |
N | System suitability test result (Requirement: %RSD < 2.0 %) |
---|---|---|---|---|
VP-ODS (5 μm, 4.6 x 150 mm) |
30,000 | 0.50 | 5,244 | tR: 0.025 % Area: 0.175 % (n=6) |
Velox C18
(2.7 μm, 4.6 x 100 mm) |
37,037
(+23 %) |
0.50 | 9,497 (+81 %) |
tR: 0.035 % Area: 0.103 % (n=6) |
0.93 | 4,466 (-15 %) |
tR: 0.084 % Area: 0.220 % (n=6) |
News / Events
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An Improvement in Preparative SFC Efficiency Utilizing Online SFE-SFC System as a “Solid Autosampler”
Preparative SFC is a groundbreaking separation and purification technology. It has a number of advantages in comparison to preparative LC including simplified posttreatment, shorter analysis times, and different separation selectivity. With preparative SFC, the injection of large sample volumes is often required in order to increase the recovered amount.
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SFC Analytical Condition Parameters is available.
The approach for the SFC analytical condition development process is basically the same as for determining analytical conditions for HPLC.
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Latest issue of Shimadzu Journal, featuring Forensics / Toxicology, has come out.
This issue focuses on "Forensics / Toxicology", an area where science and technology are particularly important and needed. It starts with two customer interviews.
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Analysis of Tea Beverages Using Catechin Analysis Kit
The catechin analysis kit, developed in collaboration with the National Agriculture and Food Research Organization (NARO), provides validated test methods for catechins in tea leaves and green tea drinks from sample pre-treatment to HPLC measurement. You can accurately quantitate the content of 11 catechins (including methylated catechins) and caffeine in tea leaves. This article summarizes the results of the catechins quantitation in several beverages: green tea, black tea, and oolong tea.
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Extraction and Fractionation of Functional Ingredients with Online SFE-SFC
This article describes a preparative online supercritical fluid extraction-supercritical fluid chromatography (SFE-SFC) system that combines an extraction step using supercritical carbon dioxide as the solvent, a separation step using supercritical carbon dioxide as the mobile phase, and a fractionation step using a preparative-scale fraction collector (FRC).
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New UF-Amino Station
UF-Amino Station, which was developed in collaboration with Ajinomoto Co., Ltd., uses a special high-speed and high-separation column and the LCMS-2020 mass spectrometer, to perform batch analyses of 38* amino acids and amino acid-related components in a mere 9 minutes.