Last update: May 9, 2022 GMT

Plunger assembly
P/N Appearance Qty Material Remarks Applicable model Links Applicable models
228-52069-44 1 Sapphire With diaphragm 40DXR
40DXS
40DX3
40BXR
40BX3
228-35009-93 1 Sapphire → External view 20AT
20Ai
optional for 10ATvp
228-20284-91 1 Sapphire 228-17019-93 with holder
→ External view
20AR
6AD
3 Sapphire → External view 20AR
6AD
228-17019-93 1 Sapphire → External view 20AR
6AD
228-32654-92 1 Zirconia With a holder *1)
→ External view
optional for 10Ai
228-32654-91 1 Sapphire With a holder *1)
→ External view
10Ai
228-35009-92 1 Zirconia *2)
→ External view
10ATvp
228-35281-91 1 Zirconia 228-35601-91 with holder and O-ring. *2)
→ External view
10ADvp
228-70428-41 1 Sapphire Mikros
228-35601-91 1 Zirconia *2)
→ External view
10ADvp
228-35601-93 1 Sapphire → External view 30ADSF
20AB
20AD
20ADXR
20ADSP
20ADnano
2010
2010HT
optional for 10ADvp
228-35281-95 1 Sapphire 228-35601-93 with holder
→ External view
30ADSF
20AB
20AD
20ADXR
20ADSP
20ADnano
2010
2010HT
optional for 10ADvp
228-51269-41 1 Sapphire → External view 20AP
228-52069-94 1 Sapphire With diaphragm
→ External view
30AD
228-35281-97 1 Sapphire With diaphragm
→ External view
2040
2030
228-16837-91 1 Sapphire With a holder *1)
→ External view
8A

*1) This plunger has a plunger fixing holder as an integrated part and is finely adjusted. A plunger assembly is not provided as a single part.
*2) The color of the current plunger is white. Plungers provided up to April 2002 were cream-colored and ones provided up to July 1998 were white.

Changing a material of a plunger seal may increase the service life depending on the type of the mobile phase used.

Plunger Seal

Changing a material of a plunger seal may increase the service life depending on the type of the mobile phase used. When changing a material of a seal, seats and other parts are also necessary.
For details, click here.

■ Material Indication
GFP, GF: Reinforced PTFE (PTFE including additives), PE: UHMWPE (Ultrahigh molecular weight polyethylene)

P/N Appearance Qty Material Remarks Applicable model Links Applicable models
228-52711-93 1 PE with Backup Ring 40DXR
40DXS
40DX3
40BXR
40BX3
30AD
2040 Plus
Mikros
228-51266 1 GFP 20AP
228-35146 1 GFP 2030
2010
2010HT
20AD
20AB
20ADVP
20ADnano
opitional for 2040
3 GFP 2030
2010
2010HT
20AD
20AB
20ADVP
20ADnano
opitional for 2040
228-35145 1 GFP 20AT
10ATVP
3 GFP 20AT
10ATVP
228-32628-91 1 PE 2040
30ADSF
20ADnano
20ADXR
20ADSP
optional for 2030
optional for 2010
optional for 2010HT
228-21975-91 1 PE 20AR
20Ai
10Ai
6AD
optional for 10ATVP
3 PE 20AR
20Ai
10Ai
6AD
optional for 10ATVP
228-18092 1 GF 8A
3 GF 8A

Notes for Changing a Material of a Plunger Seal

When changing a material of a plunger seal, parts other than the seal are also necessary.
See the details below.

Seal Material Other Than LC-10AT/LC-10ATVP LC-10AT/LC-10ATVP
GFP→PE
GF→PE
PE seal kit (P/N 228-33233-91)
A set including the following parts
  • PE seal (P/N 228-32628-91)
  • PVDF seat (P/N 228-33760-92)
  • Cushion for pump head (P/N 228-33230)
Two types of seats (P/N 228-32325-01 and 228-32401)
For the LC-10ATVP, the rinsing seal also needs to be changed.
→Rinsing seal for the LC-10ATVP
PE→GFP GFP seal kit (P/N 228-35349-91)
A set including the following parts
  • GFP seal (P/N 228-35146)
  • PVDF seat (P/N 228-33760-92)
  • Cushion for pump head (P/N 228-33230)
Seal cap (P/N 228-35804)*2)

*2) For early LC-10ATVP models, P/N 228-35147 (metallic) was used. Currently 228-35804 (trifluoroethylene) is used.

Rinsing Seal of the LC-10ATVP

When changing a material of a plunger seal from GFP to PE or changing the rinsing seal for the LC-10ATVP manufactured in June 1998 or earlier to the current seal, the rinsing seal and mounting parts for it also need to be changed.

Plunger Seal

When Changing a Material of a Plunger Seal from GFP to PE

When changing a plunger seal with one made of PE, the rinsing seal and mounting parts for it also need to be changed. The following additional parts are necessary.

  • Rinsing seal P/N 228-28499
  • Seal holder P/N 228-35816-91
  • Ring holder P/N 228-39109-01
Plunger Seal

When Changing the Rinsing Seal Used on the LC-10ATVP Manufactured in June 1998 or Earlier to the Current One

When changing the rinsing seal to the current one (P/N 228-35145), the holder also needs to be changed. Use the P/N 228-35946-91 seal holder assembly (with a seal).

Suction Filter
P/N Appearance Qty Remarks Applicable model Links Applicable models
228-56200-48 1 Assembly (stainless steel) 40BX3
1 Assembly (stainless steel) Mikros
1 Assembly (stainless steel) 2040
2040 Plus
2030
2030 Plus
1 Assembly (stainless steel) 20ADnano
228-45708-91 1 Assembly (stainless steel) 30ADSF
30AD
2030
2040
2010
2010HT
20ADXR
20ADnano
20AR
20AD
20AB
20AT
6AD
optional for 10ADVP
optional for 10ATVP
228-39181-94 1 Assembly (ceramic) 20ADSP
20Ai
10Ai
228-25079-91 1 Ceramic filter main unit 20Ai
10Ai
optional for 20AD
optional for 10AB
optional for 20AT
optional for 10ADVP
optional for 10ATVP
228-20031 1 Filter main unit, ESKA-P5375C (stainless steel) 20AP
8A
228-18740-95 1 PE optional for 20Ai
228-17421-91 1 Assembly (stainless steel) 8A
228-17421-42 1 20AP

On the assembly, the filter main unit (stainless steel or ceramic), tube, coil spring, ferrule, bush, and other parts are mounted.

Line Filter
P/N Appearance Qty Remarks Applicable model Links Applicable models
228-57501-92 1 40DXS
40DX3
40BX3
228-70394-42 1 Mikros
228-58173-42 1 with Certificate of Materials 30ADSF
228-35871-99 1 30AD
228-35871-96 1 Housing, integrated with a stainless steel filter 2030
2040
2010
2010HT
20ADXR
20ADnano
20AR
20AD
20AB
20AT
optional for 10ADVP *2)
optional for 10ATVP *2)
228-39082-91 1 Line filter, housing 10ADVP
10ATVP
228-48607-91 1 PEEK frit 20ADSP
20Ai
10ADVP *2)
10ATVP *2)
10Ai *2)
optional for 20AD *1)
optional for 20AB *1)
optional for 20AT *1)
5 PEEK frit 20ADSP
20Ai
10ADVP *2)
10ATVP *2)
10Ai *2)
optional for 20AD *1)
optional for 20AB *1)
optional for 20AT *1)
228-33265 1 Line filter, housing 20Ai
10Ai
228-12642-93 1 Line filter assembly
Packing (P/N: 228-12564) is neccessary when replaced.
6AD
5 Line filter assembly
Packing (P/N: 228-12564) is neccessary when replaced.
6AD
228-18405-91 1 Line filter (with packing) 20AP
8A

*1) Former standard accessories for the LC-20AD/AB/AT
*2) On the LC-10ADvp/10ATvp, 228-35871-96 is easier to handle than 228-48607-91.

Check Valve

■ Material of the Check Valve
Unless the material is described in Remarks, the cartridge is made of stainless steel, the ball is made of ruby, and the seat is made of sapphire.

Name P/N Appearance Qty Remarks Applicable model Links Applicable models
Check Valve IN 1 40DXS
40DX3
40BX3
Check Valve IN 228-39093-84 3 10ADVP
Check Valve IN 228-20256-91 1 →Exploded view 8A
Check Valve IN 1 The cartridge is made of PEEK. 20Ai
10Ai
Check Valve IN 3 The ball and seat are ceramic
→Exploded view.
optional for 10ATVP *1)
Check Valve IN 228-35721-91 1 The ball and seat are ceramic
→Exploded view.
optional for 10ATVP *1)
Check Valve IN 3 →Exploded view 10ATVP
Check Valve IN 228-32166-91 1 →Exploded view 10ATVP
Check Valve IN 3 The ball and seat are ceramic. optional for 20AD
optional for 20ADXR
optional for 20AB
optional for 20ADSP
optional for 20ADnano
optional for 20AT
Check Valve IN 228-35719-92 1 The ball and seat are ceramic. optional for 20AD
optional for 20ADXR
optional for 20AB
optional for 20ADSP
optional for 20ADnano
optional for 20AT
Check Valve IN 228-39093-92 1 →Exploded view 10ADVP
Check Valve IN 1 Primary Check valve cartridge Mikros
Check Valve IN 228-37149-92 1 Check valve cartridge, DIV
→Exploded view
2010
2010HT
Check Valve IN 228-48249-42 1 In the case of the LC-20AT: Lefy side when viewed from the front Reference: Illustration of LC-20AT Flow Line
→Exploded view
20AT
Check Valve IN 228-20283-91 1 →Exploded view 20AR
6AD
Check Valve IN 228-50950-41 1 →Exploded view 20AP
Check Valve IN 228-52964-95 1 →Exploded view 30AD
Check Valve IN 228-48249-97 2 →Exploded view 40D
40DXR
40BXR
2030
2040
20AD
20ADXR
20AB
20ADSP
20ADnano
Check Valve IN 228-48249-96 1 →Exploded view 40D
40DXR
40BXR
2030
2030 Plus
2040
2040 Plus
20AD
20ADXR
20AB
20ADSP
20ADnano
Check Valve IN 1 with Certificate of Material 30ADSF
Check Valve IN 1 Secondary Check valve Mikros
Check Valve IN 3 →Exploded view 8A
Check Valve OUT 1 40D
40DXR
40BXR
Check Valve OUT 1 with Certificate of Meterial 30ADSF
Check Valve OUT 228-53334-96 1 →Exploded view 40D
40DXR
40BXR
30AD
Check Valve OUT 228-45705-91 1 →Exploded view 2030
2040
20AB
20AD
20ADXR
20ADSP
20ADnano
optional for 10ADVP *2)
optional for 10ATVP *2)
Check Valve OUT 228-50955-41 1 →Exploded view 20AP
Check Valve OUT 228-37147-93 1 →Exploded view 2010
2010HT
Check Valve OUT 228-34976-91 1 →Exploded view 10ADVP
10ATVP
Check Valve OUT 228-34976-81 3 →Exploded view 10ADVP
10ATVP
Check Valve OUT 228-32798-91 1 The cartridge is made of PEEK. 20Ai
Check Valve OUT 3 →Exploded view 10Ai
Check Valve OUT 228-09054-93 1 →Exploded view 20AR
8A
Check Valve OUT 3 6AD

*1) If acetonitrile (100 % and other high concentrations) is left, a heavy dirt may remain inside the check valve, causing a malfunction. This check valve can operate smoothly even under such circumstances. When using this valve, check the flowrate. The flowrate may need to be corrected.

*2) When using on the 10ADvp/10ATvp, check the flowrate. Correct the flowrate if necessary.

Rinsing the Check Valve

Check valve tends to get dirty easily as solvents go through them. When using a check valve for a long period of time, it is recommended to rinse the valve regularly. Rinse the check valve when the operation of the instrument is stopped after analysis is finished, on weekends, or during a long no operation period.

 

Rinsing Procedures

  • Prepare a reservoir bottle containing 2-propanol (approx. 200 mL).
  • Connect a resistance tube (inner diameter 0.1 mm, 2 m long) to the outlet of the solvent delivery pump.
  • Replace the mobile phase with 2-propanol by for example using the solvent delivery pump's purge operation.
  • Set the flowrate so that the pressure is at least 10 MPa (standard: 1 mL/min).
  • Deliver 2-propanol for at least 30 minutes.

 

Flowrate Correction
On the 20A/VP series, users can correct the flowrate using the VP function.
Measure the flowrate at the flowrate to be corrected and set the value for [FLOW COMP.FACT] on the [Calibration] menu of the VP function. For details, refer to the "Calibration Support Group" section of the instruction manual.

Diaphragm, rinsing seal, bearing
Name P/N Appearance Qty Remarks Applicable model Links Applicable models
228-55272-41 2 40D
40DXR
40DXS
40DX3
40BXR
40BX3
30AD
2030
2040
2040 Plus
228-32784-91 2 White, PTFE (polytetrafluoroethylene), more solvent-tolerant and mechanically stronger than 228-24311-01 30ADSF
20AB
20AD
20ADXR
20ADSP
20ADnano
2010
2010HT
10ADVP
228-24311-01 1 Black, Viton® (fluoroelastomer), low solvent-tolerant *1) usable for 10ADVP
228-24311-84 10 Black, Viton® (fluoroelastomer), low solvent-tolerant *1) usable for 10ADVP
228-51263 1 20AP
228-35935 1 Polyethylene, white (translucent) *2) 20AT
20Ai
10ATVP
228-28499 1 Polyethylene, white *2) 10Ai
3 Polyethylene, white *2) 10Ai
228-18306 1 PTFE (polytetrafluoroethylene), gray 8A
5 PTFE (polytetrafluoroethylene), gray 8A
228-20282 1 PTFE (polytetrafluoroethylene), gray 20AR
6AD
1 PTFE (polytetrafluoroethylene), gray 20AR
6AD
Rising Seal 1 Mikros

*1) Viton®is a registered trademark of DuPont Dow Elastomers Japan.

*2) On the LC-10ATvp units manufactured in June 1998 and later, the rinsing seal 228-35935 is used. On units manufactured earlier and ones with a polyethylene plunger seal, the rinsing seal 228-28499 is used. When changing the seal to a different type, the seal holder assembly also needs to be changed. (Details)

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