Shim-pack Diol Series Analysis Examples

Calibration Curves

■Peaks
1. 
2. 
3. 
4. 
5. 
6. 
7. 
8. 
9. 
10. 
11. 
12. 
13. 
Blue dextran 2000
Thyroglobulin
y-Globulin
Transferrin
Human serum albumin
Ovalbumin
β-Lactoglobulin
Chymotrypsin
Myoglobin
Lysozyme
Ribonuclease A
Cytochrome C
Gly-Tyr
 
■Conditions    
Column Shim-pack Diol Series (500 mmL × 7.9 mmI.D., 5 μm)
Mobile Phase A) 10mM phosphate buffer solution (pH 7)
B) 0.2M sodium sulfate
Flow Rate 1.0 mL/min
Col. Temp. Ambient
Detection UV 280 nm
 

Analysis of Protein Standard

■Peaks
1. 
2. 
3. 
4. 
5. 
Glutamate dehydrogenase
Lactate dehydrogenase
Enolase
Adenylate kinase
Cytochrome C
 
■Conditions    
Column Shim-pack Diol-300 (500 mmL × 7.9 mmI.D., 5 μm)
(P/N: 228-14776-92)
Mobile Phase A) 10mM phosphate buffer solution (pH 7)
B) 0.1M sodium chloride
Flow Rate 1.0 mL/min
Col. Temp. Ambient
Detection UV 280 nm

For more applications, please click here.

Product Information

Column Stationaryphase Particle Size(µm) Dimensions(I.D. x Length, mm) P/N
Shim-pack Diol-150 Diol group 5 7.9 x 250 228-14775-91
7.9 x 500 228-14775-92
Shim-pack Diol-300 Diol group 5 7.9 x 250 228-14776-91
7.9 x 500 228-14776-92
Pre-column Diol * Diol group 10 4.0 x 50 228-16367-91

* Installed between the liquid pump and the sample injector to protect Shim-pack Diol column.