Strict Manufacturing Uniformity

Comparison of reproducibility between three batches of silica-based materials

Balance of Number of Theoretical Plates and Column Pressure

Shim-pack VP series achieves a higher number of theoretical plates while maintaining low pressure. It provides superior performance shown by NTP per 1MPa.

NTP per 1MPa
Column Size: 4.6 x 150 mm, Mobile Phase: Methanol/Water=70/30,
Flow Rate: 1.0 mL/min., Sample: Naphthalene

Excellent Peak Shape

Packing materials with less metal impurities achieve excellent peak shape of coordination compounds.

Completed end-capping achieves excellent peak shape of basic compounds.

Smooth Transfer of Methods from High-Speed Analysis to Conventional Analysis

By using the Shim-pack VP series of conventional columns in combination with the Shim-pack XR series of high-speed columns, which offer the equivalent separation performance, it is possible to perform method development via high-speed analysis, and then smoothly transfer to conventional analysis. Utilizing these columns with Nexera Method Scouting provides strong support for method development.

Conditions
Column Top:
Shim-pack VP-ODS
(150 mmL. × 4.6 mmI.D., 5 μm)
(P/N: 228-34937-91)
    Bottom:
Shim-pack XR-ODS
(50 mmL. × 3.0 mmI.D., 2.2 μm)
(P/N: 228-41606-92)
Mobile Phase A) 0.1 % formic acid
B) Acetonitrile
A/B = 8/2 (v/v)
Flow Rate Top: 1.0 mL/min
Bottom: 1.0 mL/min
 
 
 

Relative Retention Times

Peak Shim-pack VP-C8 Shim-pack XR-C8
  1. Sulfadiamine 1.000 1.000
  2. Sulfamerazine 1.252 1.238
 3. Sulfadimidine 1.537 1.509
 4. Sulfamethoxazole 2.809 2.699
 5. Sulfaquinoxaline 5.668 5.432

Analysis of Fat-Soluble Vitamin

Conditions
Column Top:
Shim-pack VP-C8
(150 mmL. × 4.6 mmI.D., 5 μm)
(P/N: 228-59927-91)
  Bottom:
Shim-pack XR-C8
(50 mmL. × 3.0 mmI.D., 2.2 μm)
(P/N: 228-59902-92)
Mobile Phase Methanol
Flow Rate Top: 1.0 mL/min
Bottom: 1.0 mL/min
 

Relative Retention Times

Peak Shim-pack VP-C8 Shim-pack XR-C8
1. Retinol 1.000 1.000
2. α±−tocopherol 1.509 1.531


 

 
 

Analysis of Fat-Soluble Vitamin

Conditions
Column Top:
Shim-pack VP-C8
(150 mmL. × 4.6 mmI.D., 5 μm)
(P/N: 228-59927-91)
  Bottom:
Shim-pack XR-C8
(50 mmL. × 3.0 mmI.D., 2.2 μm)
(P/N: 228-59902-92)
Mobile Phase Methanol
Flow Rate Top: 1.0 mL/min
Bottom: 1.0 mL/min

 

Relative Retention Times

Peak Shim-pack VP-C8 Shim-pack XR-C8
1. Retinol 1.000 1.000
2. α±−tocopherol 1.509 1.531
 

Batch Analysis of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs)

Conditions
Column Top:
Shim-pack VP-Phenyl
(150 mmL. × 4.6 mmI.D., 5 μm)
(P/N: 228-59928-91)
  Bottom:
Shim-pack XR-Phenyl
(50 mmL. × 3.0 mmI.D., 2.2 μm)
(P/N: 228-59904-92)
Mobile Phase A) 20 mmol/L Phosphoric acid (Na) buffer (pH 2.5)
B) Acetonitrile
A/B = 3/7 (v/v)
Flow Rate Top: 1.0 mL/min
Bottom: 1.0 mL/min

 

Relative Retention Times

Peak Shim-pack VP-Phenyl Shim-pack XR-Phenyl
1. Piroxicam 1.000 1.000
2. Ketoprofen 1.480 1.483
3. Diflunisal 2.233 2.172
4. Diclofenac 2.949 2.916
5. Indomethacin 3.263 3.262
6. Ibuprofen 3.470 3.501

Specifications

  Shim-pack VP-ODS Shim-pack VP-C8 Shim-pack VP-Phenyl
 Particle Size (µm)  5  5  5
 Pore Size (nm)  12  12  12
 Surface Area (m2/g)  410  410  410
 Carbon Loading  20%  12.5%  12.3%
 Pressure Tolerance (MPa)  Approx. 20  Approx. 20  Approx. 20
 Pore Volume (mL/g)  1.25  1.25  1.25
 End-capping  Yes  Yes  Yes
 Bonding Type  Monomeric  Monomeric  Monomeric
 pH range  2-7.5  2-7.5  2-7.5
 USP Code  L1  L7  L11

* Appearance and specifications are subject to change without notice.