Nexera lite inert - Features

Antibody aggregates indicate the immunogenic potential. In addition, aggregates can result in lower purification efficiency and lower production quantities of antibodies, which are the main active ingredient in antibody drugs. Therefore, antibody drug aggregate analysis is an important process in the manufacturing and quality control of biopharmaceuticals. Using a Nexera lite inert system ensures reliable data can be acquired without worrying about protein adsorption or the risk of corrosion by high salt concentrations.

In addition to a general-purpose absorbance detector, Nexera lite inert systems support using a metal-free inert cell with a fluorescence detector, expanding their use to a wider range of applications. The figure on the right is from an example of using gel filtration chromatography to evaluate the molecular weight distribution of heat-treated membrane proteins that contain green fluorescent protein (GFP). It shows that structural changes due to the heat treatment temperature can be detected by fluorescence detection.

Using a combination of multiple detectors and fraction collectors offers the feexibility to purify samples based on the specific characteristics of target compounds. For example, by using both an absorbance detector and fluorescence detector, antibodies with and without fluorescent labeling can be monitored at the same time. Furthermore, by using an FRC-10A fraction collector to ensure reliable preparative separation of only target components, fluorescently labeled antibodies can be used directly in the next process step.