Nexera UC - Features

Ideal for simultaneous analysis of compounds with a wide range of polarities

SFC can achieve a wide variety of separation patterns. This allows comprehensive analysis of compounds over a wide polarity range not previously possible with HPLC.　Different separation methods are generally used for fatty acids, which are typically analyzed by GC, and glycerides, which are typically analyzed by HPLC. However, because supercritical carbon dioxide has properties similar to hexane, SFC is well-suited for analyzing compounds with different polarity, providing an ideal solution for the simultaneous analysis of fatty acids and glycerides.

Simultaneous analysis of fatty acids and glycerides by SFC

Sensitivity results from different separation modes in HPLC vs SFC

Supercritical CO₂ has unique properties different from liquid. Using SFC in front of a mass spectrometer offers greater sensitivity than achieved with LC/MS/MS.

Comparison of peak intensity detected by LC/MSMS and SFC/MS/MS.
(Sample: Prostagrandin D2 10 pg)

Automatically performs a variety of method scouting processes

The high-speed performance of SFC can shorten the time required for method scouting. The system automatically generates a large number of methods by utilizing combinations of up to 12 columns, four modifiers, and different ratios of modifiers to mobile phase. With UHPLC/SFC Switching system, screening by UHPLC and SFC can be carried out in the same batch. Moreover, Method Scouting Solution, optional software for method scouting, makes it easy to set up multiple conditions.

A screen shot of Method Scouting Solution for Nexera UC user interface.

Chiral analysis with Nexera UC Chiral Screening System

CHIRALPAK^® Series and CHIRALCEL^® Series columns (Daicel Corporation) for chiral analysis are capable of resolving a wide variety of compounds by showing complementary separation targets. The combination of the Nexera UC Chiral Screening System and these columns simplifies method scouting for chiral analysis.

 

Automated screening by automatically switching between mobile phases and columns

The high speed of SFC is well suited to saving time during separation of optical isomers and simultaneously analyzing a variety of compounds, but developing analysis methods for SFC is tedious and time-consuming. The Nexera UC chiral screening system can significantly improve screening efficiency by automatically switching between up to 12 columns and 7 types of modifiers, and can even adjust the blending ratio for those conditions. Furthermore, by using a UHPLC/SFC switching system*, screening can successively switch between UHPLC and SFC analysis modes.

* The UHPLC/SFC switching system has a maximum operating pressure of 66 MPa.

 

Easily create and execute analysis schedules for screening

A large number of method files or analysis schedules can be quickly created by executing the 4 steps listed below from ❶ to ❹. The ability to select the mobile phase and column with a single click can improve operating efficiency and reduce errors. The screening system includes an auto-stop function that automatically stops searches if condition settings that satisfy specified criteria are found.

 

Upgrade to Analytical Fraction System

By adding a fraction collector to the analytical scale SFC, small-volume fractionation is also possible. A complete workflow can be carried out seamlessly on one system, from method development to fractionation.

 

In preparative SFC, one factor that results in lower recovery rates is increased scattering of the eluent when the CO₂ returns from a supercritical to a gaseous state. The Nexera UC Prep’s patented gas-liquid separator, the LotusStream™ separator, successfully reduces sample dispersion and carryover, while also achieving high recovery rates. It allows fractionation of small volumes, even into vessels such as 1.5 mL vials. The optical isomers of linalool were fractionated with high purity.

 

Nexera UC on-line SFE-SFC is a revolutionary system that combines on-line supercritical fluid extraction (SFE) and SFC in a single flow path (patented technology). Target compounds are extracted from solid samples and then automatically transferred to SFC analytical system so that no human intervention is required. The Nexera UC on-line SFE-SFC system reduces the time for pretreatment of samples and acquires highly accurate data. In addition, off-line SFE can simplify pretreatment over existing solvent extraction methods.

Comparison of QuEChERS sample preparation and Nexera UC in the analysis of residual pesticides

A typical sample preparation takes 35 minutes and requires several manual steps. With Nexera UC, the same sample can be ready for on-line SFE/SFC analysis in as little as five minutes with only a few simple sample preparation steps.

* "Miyazaki Hydro-Protect", Patented in Japan No.3645552

Customized modules for Nexera UC based on Nexera technology

Supercritical Fluid Extraction Unit SFE-30A

This unit operates at a maximum temperature of 80℃ to allow faster and more complete extractions. Five types of extraction vessels (two sizes (5 mL, 0.2 mL)made of PEEK(maximum pressure 20MPa), three sizes (10 mL, 5 mL, 0.2 mL) made of SUS) can be chosen based on the sample and sample amount.

 

CO₂ Solvent Delivery Unit LC-30AD_SF / Back Pressure Regulator Unit SFC-30A

Stable baseline with low pulsation is realized by LC-30AD_SF with a built-in cooler for pump heads, which delivers mobile phases up to 5 mL/min at pressures up to 66 MPa. The low dead volume of SFC-30A (0.7 μL) allows a mass spectrometer to be directly connected to the SFC system without splitting so that higher sensitivity can be achieved.

 

Comparison of costs and consumption of organic solvent for a single analysis by conventional normal-phase HPLC vs. SFC is shown below. By using SFC, the total cost of analysis is reduced by 87.6% and the consumption of organic solvent is reduced by 94.2%.