Establishment of a Method for Direct Analysis of the Mouse Liver Metabolome Using the DPiMSTM-8060

Introduction

In the analysis of endogenous metabolites (metabolome analysis), it is difficult to perfectly remove biases caused by pretreatment and sampling. Therefore, in order to accurately grasp the changes in the metabolome of a biospecimen, the establishment of a method for direct analysis of the metabolome is indispensable. Probe electrospray ionization (PESI) is a new direct ionization method in which an ultrafine and minimally invasive probe is used for sampling. Acquired samples are ionized by applying a high voltage to the probe tip and therefore components can be analyzed without using a chromatograph. By using the DPiMS-8060 probe electrospray ionization tandem mass spectrometer, which combines PESI with tandem mass spectrometry, direct analysis of the metabolome of a biospecimen is possible. This article introduces a method established to directly analyze the metabolome of a tissue sample (intact metabolome analysis) using a PESI tandem mass spectrometer together with the application of the method to the metabolome analysis of CCl₄-induced acute liver failure model mice.

January 16, 2019 GMT