Glycerophospholipids Analysis by Comprehensive HPLC Coupled with a Triple Quadrupole Mass Spectrometer

Introduction

Glycerophospholipids (GPLs) are the major component of biological membranes. They can not only act as a barrier from the external environment, but can also play a key role in a variety of biological processes including membrane trafficking and signal transduction. Thus, analysis of GPLs is one of the most important studies in the metabolomics field. Although reversed phase (RP) HPLC coupled with electrospray ionization (ESI) MS/MS is an effective strategy for lipidomics, there is still room for further improvement of the analytical methods. One drawback to performing determination of GPLs is ion suppression caused by co-eluting compounds. To obtain reliable results, complete separation of target GPLs by comprehensive HPLC with ESI-MS/MS is an effective strategy.

July 1, 2014 GMT