Comprehensive 2D-LC is a new analytical methodology combining two independent separation modes orthogonally, greatly increasing separation efficiency.
Nexera-e
Comprehensive Two-Dimensional Liquid Chromatograph
The comprehensive 2D-LC methodology is a paradigm shift in liquid chromatography separation analysis. By combining two independent separation modes orthogonally in combination with a dual-loop/dual-valve alternate switching design, the highest possible peak capacity is achieved for LC chromatography separation. The new Nexera-e enables separation of even the most complex mixtures, providing a new level of knowledge and understanding of sample analytes. This is especially beneficial to the analysis of pharmaceutical impurities, proteolytic digests, food extracts, natural products and synthetic polymers.
Separation far exceeding conventional 1D-LC
How many peaks are co-eluted under one peak? The more complex the sample, along with a high percentage of similar compounds, means there is a high probability of co-eluting compounds found under single peaks.
Even though the co-eluting compounds may be unable to be separated by mono-dimensional separation, the orthogonal two-dimensional system will provide the best possible result.
Comparison of the separation of a traditional Chinese medicine sample using Nexera-e and
a conventional 1D-LC system
As shown, the figures the unseparated compounds with the mono-dimensional separation system can be separated with the two-dimensional system. Moreover, in the same analysis time, Nexera-e shows more than 200 peaks versus only 74 peaks in the conventional 1D-LC system.
* Appearance and specifications are subject to change without notice.
Features
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On a two-dimensional map, since each 1st-dimensional axis and 2nd-dimensional axis are set according to different separation conditions, the physical properties of compounds and their position on a map correlate. -
Comprehensive 2D-LC requires excellent performance from each component. For example, the 2nd-dimensional separation system requires ultra-first gradient analysis with a high flow rate and ultra-high pressure. -
Acquired data is converted to two-dimensional contour plotting using ChromSquare, the software for comprehensive 2D-LC analysis.
Movies
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Nexera-e (Comprehensive Two-Dimensional Liquid Chromatograph)
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Comprehensive 2DLC: Concepts, Potential, Applications
News / Events
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Don’t miss out! Join our latest webinar collaborated with SelectScience on June 28, 2022.
“Dual deconvolution of LC-PDA-MS data: A fast-track approach for chemical composition and impurity analysis”
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Shimadzu has released the LCMS-2050 High-Performance Liquid Chromatograph Mass Spectrometer.
The LCMS-2050 single quadrupole mass spectrometer combines the user-friendliness of an LC detector with the excellent performance of MS to provide a complete package of easy to use high-level performance and compactness.
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LabSolutions CS has been upgraded to Ver. 6.115.
LabSolutions CS features an innovative operating environment and provides complete data management to ensure secure information in networked laboratories.
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Shimadzu has released the CoreFocus Library.
CoreFocus Library is a digital library where you can find and learn different methods for analyzing your target compounds.
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Shimadzu has released the Nexera XS inert.
The Nexera XS inert system offers the ideal solution for the separation of biomolecules by combining the elevated pressure tolerance of a UHPLC system with complete inertness of the sample flow path, ensured by the absence of wetted metal surfaces and offering ultra-high resistance to corrosion.
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Shimadzu has released Method Development System
LabSolutions MD uses an “Analytical Quality by Design” (AQbD) approach for achieving efficient method development by designing analysis methods based on science and risk. All workflow steps can be completed using LabSolutions MD, including analyzing samples using the experimental design, building a design space by using the analytical results, and evaluating robustness after deciding the optimal analytical conditions.