High Sensitivity Analysis of Aldosterone in Human Serum Using LCMS™-8060NX

Introduction

This report introduces an example of high sensitivity analysis of aldosterone in serum using LCMS-8060NX, a triple quadrupole mass spectrometer. Calibration curves prepared using standard samples showed linearity in the concentration range of 0.5 - 10000 pg/mL (R2 =0.9998), with %RSD of concentration of 3.96% at the lower limit of quantification and accuracy at each calibration point ranged from 95.58 - 109.84%. Furthermore, the accuracy of serum samples spiked with aldosterone (1 pg/mL) was 106%, indicating that the method used in the analysis is suitable for the actual samples.

October 22, 2020 GMT