Protein Analysis Using FTIR - Analysis on Changes of Secondary Structures in Egg White Proteins Caused by Thermal Denaturation -

Spectrophotometric Analysis

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Introduction

Application News No. A585 describes using curve fitting to analyze the secondary structures of bovine serum albumin (BSA). This article describes investigating the changes that occur in the secondary structures of proteins caused by thermal denaturation. The changes were probed based on second derivative and curve fitting of the spectra measured when egg white containing proteins abundantly was heated from 40 to 100 °C using a heatable three-reflection ATR accessory.

March 1, 2019 GMT

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