Analysis of Fat-soluble Vitamins Using the Nexera UC Supercritical Fluid Chromatograph

Introduction

Vitamins are essential micronutrients to maintain the normal functioning of organisms, but they must be ingested from food because they are not synthesized sufficiently in the body. Deficiencies of vitamins in the body cause symptoms such as disease and growth disorders. Vitamin A is associated with night blindness and skin abnormalities, vitamin D with rickets and osteomalacia, vitamin E with anemia and blood circulation disorders, and vitamin K with hemorrhaging, osteoporosis, etc. Therefore, it is very important to accurately analyze the fat-soluble vitamins in foods and supplements. The fat-soluble vitamins are broadly classified into vitamin A, vitamin D, vitamin E, and vitamin K according to their chemical structure and physiological effects. Due to the large number of isomers, good separation is required for fat-soluble vitamin analysis. Here we introduce an example of a simultaneous analysis of fat-soluble vitamins using a supercritical fluid chromatograph (SFC) and triple quadrupole mass spectrometer for detection.

May 13, 2019 GMT