Fast Profiling of 39 Bile Acids in Plasma, Urine and Feces, by Automated Extraction and LCMS-8060NX.

User Benefits

- Ready to use method for the quantification of a high number of Bile Acids in a short time, - Automated sample preparation protocol for plasma, urine and feces.

Introduction

Bile acids (BA) play an important role in the absorption of fat in the small intestine and are involved in the regulation of cholesterol metabolism through the conversion of cholesterol to BA. Primary bile acids are produced in the liver by cholesterol catabolism, most of which combine with taurine or glycine to form conjugated bile acids. Some primary bile acids are later modified by intestinal bacteria to produce secondary bile acids. Total bile acid concentration in human peripheral blood is known as a marker of liver dysfunction, and is widely measured together with blood enzyme activity (ALT, AST, etc.). On the other hand, profiling of many types of bile acids is attracting attention because it may be possible to distinguish various liver disorders by measuring multiple bile acids individually. However, it is difficult to measure fragment ions specific to the difference in structure because the colanic acid structure, which is the basic skeleton of bile acids, is not easily broken by MS/MS. In order to accurately quantify various chemically similar bile acids by simultaneous LC- MS/MS analysis, the isomers must be sufficiently separated by HPLC. In this report 39 bile acids in bio-samples (human plasma, human urine and mouse feces) were quantitatively analyzed using 10 internal standards. LC/MS/MS method package Bile Acids Ver.2 was used for the analysis. It contains optimized conditions for LC-MS/MS analysis and an automated sample preparation protocol. By rigorously optimizing the HPLC conditions, we were able to achieve both bile acid separation, high throughput and high sensitivity.

September 24, 2021 GMT