Development of an Analytical Tool Using Metabolic Maps Corresponding to 196 Eicosanoids and Related Fatty Acid Metabolites

User Benefits

- Can use the analytical tool with the metabolism map of 196 targets in “LC/MS/MS Method Package Lipid Mediator Ver. 3.” - By displaying quantitative differences in fatty acid metabolites on the metabolic map, the metabolic enzymes involved can be easily analyzed.

Introduction

Eicosanoids are lipid mediator species that are primarily part of the arachidonic acid cascade. The increasing sensitivity and high speed of LC/MS in recent years have led to the development of a method for comprehensively analyzing fatty acid metabolites of more than 100 targets, including not only arachidonic acid metabolites but also omega-3 fatty acid metabolites. The method is used to elucidate physiological functions and search for biomarkers. We have developed a method for the analysis of 196 eicosanoids, the “LC/MS/MS Method Package Lipid Mediator Ver. 3.” This article introduces an example of the analysis of metabolites detected in human plasma and serum by a newly developed analytical tool using the metabolic map display of the target 196 eicosanoids and related fatty acid metabolites. A total of 68 metabolites were detected in human plasma and serum by comprehensive analysis. Using this analytical tool, we were able to easily identify the enzymes involved in the detected metabolites.

November 26, 2021 GMT