Analysis of Eicosanoids in Blood Using the Same Sample Preparation Method as for Primary Metabolite Analysis

User Benefits

- The same sample solution can be used to analyze both primary metabolites and eicosanoids. - Good peak shapes and high sensitivity can be achieved for eicosanoids such as thromboxane that are difficult to analyze by LC-MS. - Low analyte concentrations of around 10 ng/mL can be analyzed.

Introduction

Eicosanoids are lipid mediators causing physiological processes such as inflammation and blood coagulation. In vivo, eicosanoids are synthesized from arachidonic acid in response to stimuli such as physical trauma or stress. Eicosanoids are considered as important as primary metabolites for elucidating physiological function and searching for biomarkers. This study used derivatization including methoximation and trimethylsilylation to quantify seven eicosanoids: prostaglandin E₂ (PGE₂), 11β-prostaglandin E₂ (11β-PGE₂), 8-iso-prostaglandin E₂ (8-iso-PGE₂), 6-keto-prostaglandin F_1α (6-keto-PGF_1α), prostaglandin F_2α (PGF_2α), prostaglandin D₂ (PGD₂), and thromboxane B₂ (TXB₂) using the GCMS-TQ8050 NX.

April 5, 2022 GMT