Optimizing Analytical Conditions for Lipid Analysis by SFC and Its Application for Precise Preparative Chromatography – Case Study of Diglyceride Isomers –

Introduction

Supercritical fluid chromatography (SFC) provides a unique separation behavior for relatively hydrophobic components. Components and columns exhibit complex interactions in supercritical carbon dioxide, which has a high diffusion coefficient. Another key benefit of supercritical carbon dioxide is that it can significantly reduce the volume of fractions by quickly evaporating during preparative chromatography. That means SFC enables both precision separation and efficient preparative fractionation at the same time. This report describes the use of SFC for the analysis of lipids in the presence of multiple structural isomers. SFC could also find applicability in lipidomic research and other applications.

August 8, 2022 GMT