Visualization of Glutamate Decarboxylase Activity Localization in Germinated Legume Seeds with the iMScope™ QT

Introduction

In vivo enzymatic reactions are normally detected by reacting the enzyme with a substrate, eliciting a color reaction with the resulting reaction products, and measuring absorbance or another indicator. Conventional detection methods require a primary reaction between the enzyme and substrate and a secondary reaction to produce color, while in more recent methods, these reactions are carried out on the tissue surface to visualize enzyme activity localization. This article describes a new enzyme histochemical method of detecting in vivo enzymatic reactions. using the high spatial resolution mass spectrometry imaging capabilities of the iMScope QT imaging mass microscope.

February 17, 2023 GMT