Method development and Identification of Triacylglycerols species with Supercritical Fluid Chromatography and Method Scouting Software

User Benefits

- Analytical SFC may provide shorter analytical time than conventional normal phase HPLC. - Method optimization for various analytical parameters can be achieved by Method Scouting Solutions.

Introduction

Triacylglycerols (TAGs), which are the main constituents of natural oils and fats, compose of three fatty acids and glycerol linked with ester bonds. Analyzing TAGs is essential to evaluate the quality and functionality of oils and fats. However, chromatographic separation by typical methods that include GC and HPLC might be challenging due to a large number of TAGs species. Supercritical Fluid Chromatography (SFC) is known to show similar separation to normal phase LC and by changing some parameters, has the potential capability to show superior performance for the analysis of TAGs as compared to the typical method.

March 8, 2023 GMT