Rapid Mapping of Imipramine, Chloroquine, and Their Metabolites in Mouse Kidneys and Brains Using the iMScopeTM QT

Introduction

Mass spectrometry imaging (MSI) is a well-known technique for the non-labeling visualization of analytes in biological samples. In this study, we applied a newly developed atmospheric pressure matrix-assisted laser desorption ionization-MSI known as the iMScope QT to rapid (up to 32 pixels/sec) mapping of imipramine, chloroquine, and their metabolites in C57BL/6 male wild-type mice. It revealed region-specific localization of both drugs and their metabolites in the mice’s kidneys and brains. Detailed localization information of these drugs and their metabolites observed in our study can aid in understanding clinically-relevant properties, efficacy, and potential side effects of these drugs. Our study also revealed the potential of the iMScope QT for the rapid mapping of small drugs and their metabolites present in the biologicalsamples.

March 28, 2023 GMT