Phospholipid Profiling Method in Human Plasma Using the Shim-pack Scepter Claris C18

User Benefits

- The MRM Library for Phospholipid Profiling enables the reliable identification of peaks of actual samples without the use of reference standards, allowing users to develop their own analytical methods. - With the Shim-pack Scepter Claris C18 column, analysis of phospholipids in human plasma can be performed in 20 minutes. - It enables comprehensive quantitative profiling of 162 human plasma phospholipid compounds. - Minimizing the number of MRM transitions enables quantitative profiling of human plasma phospholipids with good repeatability.

Introduction

Quantitative profiling of phospholipids in biological samples is a promising technique for discovering biomarkers and analyzing disease mechanisms. Phospholipids have diverse structures, so the number of phospholipid compounds detected varies depending on the sample. The MRM Library for Phospholipid Profiling contains a total of 1969 MRM transitions to monitor phospholipid polar head groups and fatty acids as product ions, so users can select the MRMs required for phospholipid analysis of samples. Moreover, by monitoring the product ions of both polar head groups and fatty acids, it is possible to obtain a reliable estimate of phospholipid structures even without the use of a reference standard, enabling users to create their own phospholipid profiling methods. In this article, we describe the development of a human plasma phospholipid profiling method using the Shim-pack Scepter Claris C18. Using this method, we detected 162 phospholipid compounds in a human plasma sample. By minimizing the number of MRM transitions, we also improved the repeatability of peak area values.

July 8, 2025 GMT