Automated Preparative Purification Using Nexera UFPLC —High Concentration Collection via an Online Trap Column—

User Benefits

- By repeatedly loading fractions onto the trap column, high-concentration collection is achieved. - Because the collected fraction is organic solvent–rich, post-purification drying and powdering can be shortened. - The target compound can be collected at high concentration regardless of peak shape.

Introduction

Preparative LC is widely used across the pharmaceutical, food, and chemical industries for applications such as purification of target compounds from mixtures, screening of active components in natural products, and structural elucidation of impurities and unknown compounds. In Application News 01-00937, a single analytical / preparative convertible LC-MS system was introduced to streamline the preparative workflow, including optimization of separation conditions at the analytical scale, scale-up and fractionation, and confirmation of purity and recovery. However, because target compounds are collected in a state diluted with mobile phase, the subsequent drying and powdering steps remain time-consuming. The ultra fast preparative and purification LC system “Nexera UFPLC” is a proprietary system equipped with functions that automate processes associated with preparative purification, including concentration and purification in addition to fractionation. This article presents a case in which Nexera UFPLC enables concentration of the target compound on an online trap column and its collection at high concentration in an organic solvent. This approach is expected to significantly reduce the effort required for post-purification drying and powdering steps.

May 7, 2026 GMT