UHPLC Method for Sensitive Automatic Analysis of Thirty-Seven D/L-Amino Acids and for Liquor Profiling

User Benefits

- Enables high-sensitivity simultaneous fast analysis of thirty-seven D/L-amino acids using simple operations. - Good reproducibility of analytical results can be achieved by keeping the derivatization reaction time constant. - Statistical analysis software enables easy multivariate analysis of multiple samples.

Introduction

Recent advances in analytical techniques have revealed the presence of D-amino acids in fermented foods and biological samples and clarified their role in the taste, preservation, and aroma of foods, and as potential disease biomarkers. Therefore, the demand for D/L separation of amino acids is increasing. Because D-amino acids are only present in trace amounts in fermented foods and biological systems, unlike L-amino acids, it is necessary to determine the concentration of each D-amino acid by separating them from the high concentrations of L-amino acids. A comprehensive method for quantitating the thirty-seven D/L-amino acids was previously reported, in which two chiral derivatizing reagents were employed to provide different diastereomers for each amino acid, with the instrument automatically switching between two separation methods to analyze each sample twice. This article introduces an improved method that employs a single chiral derivatizing reagent to enable simultaneous analysis of D/L-amino acids in a shorter time.

June 2, 2026 GMT