Chlorophyll Quantitative Measurement: Using Equations from Japanese Water Standard Test Methods

User Benefits

- In quantitation/photometric mode, LabSolutions UV-Vis can now be configured with coefficients on a per-sample basis when calculating concentration. - Quantitation/photometric mode has been updated to support a wider range of mathematical functions (power, square root, common logarithm, and natural logarithm).

Introduction

The tap water we use every day is subject to water quality controls that include Japanese water standard test methods. These water standard test methods are mainly performed on GCMS, LC-MS, and HPLC systems, although absorption spectrophotometry using a spectrophotometer is used to test for some substances. The quantification of concentration is typically determined based on calibration curves, although some substance concentration is determined based on complex equations. These complex equations can sometimes require the user to manually transfer measurement data into an Excel sheet. The risk of human error associated with this manual transfer can be avoided if the software being used to control the analysis instrument can also be configured to perform these complex equations. The updated LabSolutions UV-Vis Ver. 1.21 now offers an even greater set of features for configuring highly complex equations. This article describes using equations in LabSolutions UV-Vis to calculate the concentration of chlorophyll as outlined in Japanese Water Standard Test Methods III: Organic Materials.

August 20, 2026 GMT