Simplified Mass Measurement of Chemically-Modified Antibodies: Determination of the Presence of theNumber of Modifications Using a Linear BenchtopMALDI-TOF MS

Introduction

Antibody drug conjugates (ADC), a type of pharmaceutical composed of an antibody bound to a drug, appeared in the 2000s with the expectation they would serve as more effective anti-cancer drugs than previous small-molecule pharmaceuticals through the combination of the antibody's high selectivity and the availability of a small- molecule drug. With a small number of products already available in the marketplace, the degree to which and where binding occurs are important characteristics to determine quality when compounds are artificially bound to proteins, as in the case of ADCs. This article introduces an example of analyzing the pseudo ADC, which was created by artificially binding low- molecular compounds to a standard research antibody, using a benchtop MALDI-TOF MS.

March 13, 2019 GMT