Micro-Volume Measurement of Food Dye and Amino Acid Compound

Introduction

A UV-Visible spectrophotometer is used for the quantitative and the qualitative analysis of a substance in various fields, in which food and environmental fields need a micro-volume measurement to reduce the amount of waste solution after the measurement. Pharmaceutical, chemical and life science fields also require it because a sufficient amount of a sample used for a measurement can not be collected in same cases. In the case of a cell with a conventional quadrangular shape, its width or height is decreased to reduce the volume keeping the optical path of 10 mm and such a cell allows sample quantities of a few tens of microliters to be measured. In this report, the micro-volume measurements of food dyes and amino acid compounds using the TrayCellTM (Hellma Analytics) and the Nano Stick (SINCO Inc.) which are micro- volume cells and need sample quantities of only a few micro litters for a measurement and the UV-1900 UV-VIS spectrophotometer are demonstrated.

June 1, 2018 GMT