Analysis of Glycolysis Metabolites in Human Embryonic Stem Cells using GC-MS/MS

The analysis of metabolomes, such as when searching for disease biomarkers, is performed in many areas in the medical field, whether it be for fundamental research or for clinical studies. Single quadrupole GC-MS systems are widely used for the analysis of metabolomes, since they are capable of superior chromatographic separations and offer stable analysis.
On the other hand, biological samples include numerous metabolites and complex matrices, which can sometimes make separations using single quadrupole GC-MS difficult. Since triple quadrupole GC-MS/MS performs MS separation two times, the effects of the interfering components can be eliminated, thus making the analysis of a variety of metabolites possible.