New Data Processing Method for Photodiode Array Detectors
Principle and Overview of Intelligent Peak Deconvolution Analysis (i-PDeA II)
An i-PDeA II (Intelligent Peak Deconvolution Analysis II) data analysis technique was developed for extracting target peaks from unseparated peaks by analyzing photodiode array (PDA) detector data using the chemometrics multivariate curve resolution alternating least squares (MCR-ALS) technique. The i-PDeA II function can separate peaks for multiple components in absorption spectra and chromatograms by simply specifying the wavelength and time ranges. The i-PDeA II function can be used to identify spectra and quantitate peaks after separation of individual components, even for difficult-to-separate peaks for which a standard sample cannot be prepared. Furthermore, because i-PDeA II separates peaks based only on differences in spectral shape, it can also be used to separate and quantitate peaks for co-eluted isomers. This report explains the principle used by the i-PDeA II technique to separate peaks, describes an example of using i-PDeA II to analyze a sample with isomers of three components, and evaluates the spectral identification and quantitation performance.
- Content Type:
- Application
- Document Number:
- C191-E042
- Product Type:
- Liquid Chromatography
- Keywords:
- Photodiode array detector, chemometrics, MCR-ALS, and LabSolutions, LC-2030
- Language:
- English
- File Name:
- jpl217011.pdf
- File Size:
- 658kb