Food fraud is a problem on global scale, especially the meat fraud. Such as, horse meat was added in the beef food, and pork was used as beef. Consumers and the food industry are increasingly aware of this problem, and the specific detection of amounts of certain meat species in processed food is still problematic. Presently, ELISA and PCR methods are routinely used for the species authentication. As we know, The ELISA method often results in false positive or false negative results, and the PCR method is not so accuracy for DNA being prone to degradation.
So the characteristic peptides multiple reaction monitoring (MRM) method by LC-MS/MS is a good choice for the qualitative and quantitative analysis of meat authentication.