Genome Editing and Creating Mutant Strains in Medaka
In recent years, various genome editing technologies have been developed, such as the TALEN (Transcription Activator-Like Effector Nuclease) and CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein) systems, which allow DSB (Double-Strand Breaks) to be introduced at any desired sequence in the genome. The two are fundamentally different. TALEN is an artificial nuclease of chimeric protein which has a nuclease domain. CRISPR/Cas systems utilize an RNA-guided RGEN (RNA-guided endonuclease).1,2,3) Following those developments, a broad variety of tools based on such technology have been developed, and advancements are being made day by day.
- Content Type:
- Application
- Document Number:
- LAAN-C-XX-E020A
- Product Type:
- Life Science Lab Instruments, Electrophoresis, Chemical Inkjet Printer
- Keywords:
- Heteroduplex mobility assay (HMA) method, polyacrylamide gel electrophoresis, microchip electrophoresis system, TALEN (Transcription Activator-Like Effector Nuclease) , CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein) systems, RNA-guided RGEN (RNA-guided endonuclease), Pharmaceutical, Life Science, Genomics, Genetics, DNA sequencing, MultiNA
- Language:
- English
- File Name:
- jpu216001.pdf
- File Size:
- 2,566kb