CellTune Ver.2 - Applications

Software for Optimizing Cell Culture Conditions

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Interactive Optimization with Media Blending Kit
Increased Antibody Production Quantities by About 80%*

* The results shown are from this specific example and are not guaranteed as product performance.

CellTune was used to improve the base culture medium composition for antibody production. To optimize the composition, the following media blending kit explored and optimized the mixture ratio of eight types of supplements in one type of base medium. With culture performance based on antibody production quantity, three rounds of sequential optimization increased the score by about 80 %, which confirmed that the medium composition could be efficiently improved.

Media Blending Kit

  • The media blending kit was designed to create diversity in medium composition by allowing users to add any desired amount of supplements to a base medium. Since optimization can be achieved simply by adding supplements to the medium, the kit supports quick and easy workflow improvements.
  • Media Blending Kit

    Media Blending Kit

Designing Experiments with Constraints

The constrained Bayesian optimization function was used to output a design of experiment that maintains a constant total quantity of the base medium and supplements. Using the resulting design of experiment, antibodies were cultured using different blends of the base medium and supplements.

Designing Experiments with Constraints

Visualization of Sequential Optimization Results

The visualization function was used to show how much sequential optimization improved culture performance.
The results confirmed that multiple rounds of optimization improved scores by about 80 %, relative to the control condition. The results also showed that average improvements compared to the control condition trended upward as the number of rounds increased.

Visualization of sequential optimization Results