i-series Plus - Applications

High-Performance Liquid Chromatograph

i-Series Plus Addresses a Wealth of Applications

Although it is an integrated LC system, the i-Series Plus supports a wide range of applications according to your choice of detectors. Basic systems come with the choice of a UV-VIS detector or photodiode array (PDA) detector.
You can add to this a fluorescence detector, a differential refractive index detector and a Evaporative Light Scattering Detector, as necessary. The innovative and superior performance of the i-Series Plus supports your application requirements.

Pharmaceuticals
Foods and Beverages
 Environmental
 Chemicals
 

Pharmaceuticals

The i-Series Plus supports a wide range of HPLC analyses in the pharmaceutical industry, from identification and purity testing at the drug discovery stage to impurity testing and quantitative determination at the manufacturing and quality assurance departments.

Standard performance features, such as excellent baseline stability, ultra-low carryover performance, and excellent reproducibility of sample injection, ensure stable and validated results in any analysis based on national Pharmacopeia of each country.

 

Foods and Beverages

The i-Series Plus supports a wide range of HPLC analyses in the food and beverage industry, such as active ingredient analysis and toxic substance analysis.

Systems with a PDA detector are ideal for the analysis of synthetic pigments. Qualitative analysis with a UV spectrum and sensitive and simultaneous quantitation by wavelength switching are available. By adding an optional tungsten lamp, synthetic pigments and other substances measured in the long-wavelength region can be detected with high sensitivity.

The temperature of the oven can be controlled to as high as 85°C, while still allowing effective analysis, such as that of sugars in ligand exchange mode. Adding the RID-20A differential refractive index detector enables the detection of low light-absorbing compounds such as sugar.

Furthermore, adding the ELSD-LT II evaporative light scattering detector enables detection of low light absorbing components with a gradient elution method. A remarkably wide range of applications is possible, including simultaneous analysis of multiple components.

 

Environmental

The i-Series Plus supports a wide range of HPLC analyses in the environmental industry, water, atmospheric and soil analysis.

Analyses of environmentally hazardous substances require high-sensitivity simultaneous detection. Connecting a worldclass sensitive fluorescence detector, RF-20Axs, to a system equipped with a UV detector increases detection selectivity and sensitivity, and ensures reliable detection and identification of trace-level of environmental contaminants.

 

Chemicals

The i-Series Plus supports a wide range of HPLC analyses in the chemical industry, from small molecules to polymers.


Since the i-Series Plus column oven accommodates multiple 30 cm columns, adding a differential refractive index detector enables GPC analysis of hydrophobic polymers and other materials. In addition, "LabSolutions GPC software" facilitates molecular weight distribution calculation. For trace amounts of light-absorbing additives in samples, a UV or PDA detector can be used complementarily.

Analysis of Polystyrene with Antioxidant Additive Using Prominence-i GPC System

Gel permeation chromatography (GPC) for the analysis of hydrophobic polymers has traditionally been conducted using a differential refractive index detector.
However, when UV-absorbing trace-level additives are present along with the principal synthetic polymer component, these are sometimes analyzed using a UV detector or photodiode array detector (PDA) for highsensitivity detection. However, a combination of a differential refractive index detector and UV detector makes it possible to conduct simultaneous analysis of the principle component along with any trace-level additives, and further, permits calculation of the molecular weight distribution of the polymer, confirmation of the UV spectra of minor components, and quantitation based on the calibration curve and qualitative analysis results. 
The Prominence-i Plus integrated high-performance liquid chromatograph supports connection with the RID-20A differential refractive index detector. In addition, as the column oven can house up to three 30 cm columns used for GPC analysis, applications that require a long column are also supported.

Various types of additives, including plasticizers, antioxidants, lubricants, vulcanization accelerators and flame retardants, are generally added to polymers. This content presents an analysis of Irganox 1010, a typical hindered phenolic antioxidant which is added in small amounts to polystyrene (PS).

As shown in Fig. 1, the Irganox 1010 peak was detected directly after PS, which eluted at about 7.5 minutes. The additive peak, which was barely detected by the differential refractive index detector, was detected with high sensitivity by the PDA detector by optimizing the detection wavelength, thereby permitting quantitation. Also, as shown in Fig. 2, qualitative analysis of Irganox 1010 is possible using the UV spectrum obtained using the PDA detector.

Fig. 1 Chromatograms of Polystyrene (PS) with Antioxidant

Fig. 2 Spectrum of Irganox 1010

Quantitation and qualitative analysis of the additive -containing Polystyrene

  • Fig. 3 shows the calibration curve of Irganox 1010 analyzed using the conditions of Table 1. The calibration curve, generated over a concentration range of 10 to 100 mg/L, shows excellent linearity with a coefficient of determination greater than R2=0.999. 
    From this calibration curve, the content of Irganox 1010 of Fig. 1 was calculated to be 10.8 mg/g of polystyrene (PS).
 

Fig. 3 Calibration Curve for Irganox 1010 

Distribution of Molecular Weight

  • Fig. 4 shows the molecular weight distribution curve for the additive-containing PS using the analytical conditions of Table 1. The black-colored trace plots the molecular weight data as a differential curve, and the blue-colored trace plots that data as an integral curve. The weightaverage molecular weight (Mw) and number-average molecular weight (Mn) were 26078 and 15422, respectively. In this case, the molecular weight distribution (polydispersity: Mw/Mn) was about 1.69.
 

Fig. 4 Molecular Weight Distribution Curve for PS (5 g/L, 5 μL Injected)
Black Line: Differential Curve
Blue Line: Integral Curve 

Analysis of Sugars in Juice

The new i-Series Plus integrated high-performance liquid chromatograph can be connected to the RID-10A differential refractive index detector. Since the column oven can accommodate a 30-cm column for use in sugar analysis (ligand exchange column), and the temperature can be controlled up to 85 °C, it therefore supports applications that require a long column and high column temperature.
Here, we introduce an example of sugar analysis in juices using the Prominence-i with the RID-10A.

Analysis of Sugar Standard Solution

Fig. 1 shows the results of analysis of a standard mixture of four sugars (maltotriose, sucrose, glucose, fructose) using a 10 μL injection (each at 20 g/L). For the analytical column, we used the Shim-pack SCR-101N, a specialized sugar-analysis column that supports both the gel filtration and ligand exchange modes.



Analytical Conditions
Column : Shim-pack SCR-101N (300 mm L. x 7.9 mm I.D., 10 μm)
Mobile Phase : Water
Flowrate : 0.6 mL/min
Column temp. : 80 °C
Injection Volume : 10 μL
Detection : RID-10A Polarity +, Cell temp. 40 °C, Response 1.5 sec

Analysis of Orange Juice

Fig. 2 and 3 show the chromatograms obtained from analysis of Orange Juice A and Orange Juice B, respectively. Both Orange Juice A and B were diluted with water to obtain 10-fold dilutions, respectively, and after filtering the solutions through a 0.2 μm membrane filter, 10 μL each was injected. Sucrose, glucose and fructose were detected in both types of orange juice.

 

Applications Utilizing i-Series Plus

i-Series Solutions Package

i-Series Solutions Package is a method package that encompasses pretreatment methods, analytical conditions, and printout of judgment results. The kit supports workflow automation with a CD-ROM containing optimized pretreatment methods and analytical conditions for target components, and analytical columns that allow for sample analysis immediately after purchase.

Applications Utilizing i-Series Plus

i-Series Plus as a Front-End for LCMS

 Mass spectrometers are used in a variety of sectors, from pharmaceuticals to food. i-Series Plus (LC-2030C LT) features a compact size and the ability to process multiple samples, and combining these strengths with the superior qualitative analytical performance of Shimadzu's single quadrupole LCMS-2020 produces a system that supports powerful quantitative and qualitative analyses. Amino acids are often a target of analysis in the food sector, but are difficult to detect with UV detectors due to their normally poor light-absorbing properties. Mass spectrometers can easily analyze amino acids, so a LCMS system with excellent ease-of-use can be built by combining a detector-less i-Series Plus model with the LCMS-2020.

Example Combination LC-2030C LT LCMS-2020)

Example Combination of a Detector-less Model (LC-2030C LT)
and Single Quadrupole Mass Spectrometer (LCMS-2020)

Quick Amino Acid Analysis Via Detection by Mass

In amino acid analysis via HPLC, a long analysis time is required in order to separate the amino acids. When detecting by mass, however, the analysis time can be shortened while avoiding the impact of impurities.

Mass Chromatogram of Amino Acid Standard Sample

The table of the amino acids

MS Chromatogram for a Standard Sample of 39 Amino Acids

 

● Analysis of Sweeteners Using the LCMS-2020

Low-calories sweeteners are now widely used in food products. Sweeteners exhibit distinct flavors different from sucrose and glucose, so flavors are adjusted by combining multiple sweeteners or other methods. This publication shows the results of an analysis of carbonated beverages and a mixed standard solution of synthetic sweeteners utilizing the i-Series Plus and the LCMS-2020. Sucralose is known for its poor light absorption, which makes it a difficult component to detect using a UV detector. In contrast, the LCMS-2020 can easily analyze these sweeteners.

Chromatograms for Solutions of Carbonated Beverages Diluted 400 x